Biodiversity and Conservation Nova Hedwigia Biologie in unserer Zeit Madrid Puente Biologico 3: Mycology 2: Mycologia DOI Biodiversity and Conservation online available. DOI: Ecotropica Myxotaxon Fungal Diversity Hamburger eLMagazin Sydowia Mycopathologia Marchantiopsida: Lunulariaceae. Mycological Progress 9: North American Fungi 4: 1 Biospektrum 4: Mycotaxon Mycological Progress 8: Acta Biologica Panamensis 1: Mycological Research Mycological Progress 7: Der Palmengarten Fungal Science Stapfia Mycological Progress 2: Plant Systematics and Evolution British Journal of Dermatology Antonie van Leeuwenhoek Feddes Repertorium Kirschner R, Piepenbring M A new hyphomycete genus and species with pectinate conidia from a rainforest in Panama - a living fungal fossil?
Mycological Progress 5: Piepenbring M Checklist of fungi in Panama. Piepenbring M Inventoring the fungi of Panama.
Forest Pathology Canadian Journal of Botany Fungal Science Nr. Mycological Progress 4: Studies in Mycology Piepenbring M Comparative morphology of galls formed by smut fungi and discussion of generic concepts. Piepenbring M Fungi. Piepenbring M Smut fungi Ustilaginomycetes p. Flora Neotropica Monograph Piepenbring M, Oberwinkler F Integrating morphological and molecular characteristics for a phylogenetic system of smut fungi. Mycological Progress 1: Mycoscience Piepenbring M Annotated check list and key for smut fungi in Colombia. Piepenbring M Diversity, taxonomy, and ecology of plant parasitic smut fungi in Bolivia.
Natura In: D. McLaughlin; E. McLaughlin; P. Lemke Hrsg. Systematics and Evolution. Berlin: Springer, Mycota Bd. Lichtwardt Commemoration Volume. Plant Biology 3: Perspectives in Plant Ecology, Evolution and Systematics 4: Piepenbring M New species of smut fungi from the neotropics. Ustilaginales, Basidiomycota. Kirschner R, Oberwinkler F A new basidiomycetous anamorph genus with cruciform conidia. Piepenbring M New and poorly known smut fungi in Cuba. Mycological Research : Protoplasma Piepenbring M, Bauer R, Oberwinkler F Teliospores of smut fungi: Teliospore connections, appendages, and germ pores studied by electron microscopy; phylogenetic discussion of characteristics of teliospores.
Developments in Fungal Taxonomy | Clinical Microbiology Reviews
Progress has already been made identifying a more suitable strain by isolating a strain of Neocallimastix, that is more tolerant of oxygen, changes in culture conditions and requires fewer transfers for maintenance Leis et al. The recent identification of anaerobic fungi in novel hosts and improved understanding of the production of resistant states in the future, however, may also enable other opportunities to overcome current challenges. Meanwhile, a large amount of research effort has instead focused specifically on the exploitation of anaerobic fungal enzymes in various industries.
Anaerobic fungi produce a broad range of potent polysaccharide-degrading enzymes making them of particular interest to several industries: brewing, food, textile, paper and biofuel production. The cellulolytic and hemicellulolytic capacity conferred to the anaerobic fungi by their plant cell wall-degrading enzymes is greater than some currently used commercial enzyme mixtures.
Extracellular enzyme preparations from a Piromyces strain and N. In the paper industry, anaerobic fungal cellulases and xylanases provide environmentally friendly methods to treat paper pulp. As paper processing and pulp bleaching utilises harsh conditions high temperatures and pH , rational enzyme engineering using error-prone PCR has been used to develop a xylanase, derived from Neocallimastix , which is more stable during these processes Liu et al.
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- Progress in Mycological Research - Routledge.
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Perhaps one of the most exciting research areas for the application of anaerobic fungi, however, is that of biofuel production. There has recently been a push towards developing renewable fuels from the fermentative production of ethanol from lignocellulosic agricultural residues.
This process involves both the breakdown of the lignocellulose by carbohydrate active enzymes and the conversion of these hydrolysis products into fermentable sugars. Efforts to incorporate fibrolytic enzymes from anaerobic fungi have focused on expressing a range of carbohydrate active enzymes into a number of aerobic fungal expression strains Li et al.
Currently, the dominant microbial strain for industrial ethanol production is Saccharomyces cerevisiae , however, the wild-type strain of this yeast cannot metabolise xylose and arabinose: two important pentoses released during hydrolysis of hemicellulose. This has focused efforts on genetically engineering Sacharomyces cerevisiae to facilitate the conversion of xylose to xylulose by incorporating a xylose isomerase into this organism.
Another approach that has only recently been investigated is the use of anaerobic fungi to break down lignocellulose while simultaneously fermenting the resulting sugars to ethanol Youssef et al. This approach was used with the recently sequenced Orpinomyces sp. Although this is a relatively minor amount of ethanol, this work shows that simultaneous saccharification and fermentation is possible, and future efforts can now be directed towards enhancing ethanol yield.
A promising source of renewable, environmentally friendly energy is the production of biogas from the anaerobic digestion of organic waste. However, as biogas production is an anaerobic process, the inclusion of an aerobic pretreatment step increases the overall cost of biogas production. In contrast, the direct incorporation of anaerobic fungi into these bioreactors would eliminate the requirement of an aerobic predigestion. Anaerobic fungal genomes have an extremely high AT content Brownlee, , particularly in their noncoding regions Nicholson et al.
The first study to describe genomic sequences from an anaerobic fungus Orpinomyces sp.
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OUS1 identified multiple skeletal genes, secretory pathways, transporters, as well as numerous genes encoding for central metabolic pathways e. Pyruvate formate lyase and malate dehydrogenase and enzymes for biopolymer degradation e. Since this study, the development of high throughput sequencing approaches has provided new tools and opportunities for sequencing anaerobic fungal genomes, although their application has been challenging. The use of pyrosequencing technology alone is unfeasible, due to the high adenine—thymine AT content of anaerobic fungal genomes and their prevalence of homopolymeric A and T repeats.
Furthermore, the proliferation of simple sequence repeats also complicates the assembly of anaerobic fungal genomes generated using Illumina sequencing technologies. Using a combination of Illumina Hi-seq and Sanger sequencing approaches, however, the Joint Genome Institute was the first to generate a draft anaerobic fungal genome. Despite the Piromyces sp. More recently, Youssef et al. This recently conceived approach Koren et al. A comparison of the sequencing of the two currently available anaerobic fungal genomes is shown in Table 2 , although it should be noted that neither of these genomes have been closed.
Despite this, however, genome analysis has still provided valuable insights into anaerobic fungal genomic features, metabolic capabilities and cellular processes. In particular, two key factors appear to have shaped the genome of Orpinomyces sp. Comparison of the genome sequencing of Orpinomyces sp. These features are thought to have evolved prior to fungal separation from an Opisthokonta ancestor and appear to have subsequently been lost during the evolution of Dikarya Youssef et al. In contrast, multiple features observed in the Orpinomyces sp.
Many of the unique features of the anaerobic fungal genome could be considered a consequence of their evolution in the herbivore gut over hundreds of millions of years. Several genomic features observed in the Orpinomyces sp. Orpinomyces sp. In addition to genetic drift, the anaerobic fungal genome shows evidence of multiple adaptations to improve its fitness in the anaerobic, prokaryote-dominated environment of the herbivore gut. These adaptations include the dependence on a mixed acid fermentation pathway for pyruvate metabolism and energy production, the substitution of ergosterol which requires molecular oxygen for its biosynthesis with tetrahymanol and the acquisition of many genes from bacterial donors Youssef et al.
Of these adaptations, the latter appears to have been perhaps most important in improving the plant biomass degradation capacities of this fungus. This gene acquisition strategy appears to have evolved Orpinomyces sp.
Progress in Mycological Research
To circumvent the challenges posed by whole genome assembly, transcriptome based approaches have also recently been applied to examine the metabolic and functional capacity of the anaerobic fungal genome. The first paper to use this type of approach employed a combination of transcriptomics and proteomics techniques to identify carbohydrate active enzymes that were expressed and secreted by Neocallimastix patriciarum W5 Wang et al.
Neocallimastix patriciarum was grown on a number of recalcitrant substrates to stimulate expression of cellulases, and the transcriptome then sequenced using a combination of and Illumina sequencing technologies. A total of glycoside hydrolases from 25 different GH families were identified, with a number of these enzymes displaying novel cellulase activities Wang et al.
A meta-transcriptome approach has since been used to examine the activity of rumen eukaryotic microorganism in Musk-oxen Ovibos moschatus , through targeted sequencing of the poly-adenylated mRNA extracted from rumen solids Qi et al. The lack of detection of anaerobic fungal genomic sequences in recent rumen metagenomic studies is likely a contributing factor to this observation Brulc et al. The recent application of next-generation sequencing techniques to identify anaerobic fungi has provided a great deal of insight into the phylogenetic diversity and host distribution of these microorganism.
The identification of a number of putative uncultured genera should encourage the development of novel culturing techniques, in order to attempt to isolate anaerobic fungi representative of these taxa. It is likely that these efforts will further emphasise the need for an overhaul of the taxonomy of the Neocallimastigomycota. Increased efforts to understand the functional diversity of these microorganism are beginning to suggest that our current view of anaerobic fungi in the rumen is too simplistic. Oxford University Press is a department of the University of Oxford.
It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Sign In. Advanced Search. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents. Life cycle. Significance for other gut microorganism and the host. Biotechnological applications. Conclusions and future directions. Anaerobic fungi phylum Neocallimastigomycota : advances in understanding their taxonomy, life cycle, ecology, role and biotechnological potential Robert J.
Oxford Academic. Google Scholar. Anil K. Tony M.
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